For our training purpose, 0.33 mm should be fine for the pixel size with an image spacing of 6mm from the given spacing of the images. We now attempt to perform a 3-D volume rendering as shown in the image above. Unfortunately, we encounter a volume rendering error as demonstrated in the image below. There is not much information provided by the error message as how to proceed further. So we go back and look at the original files that were imported into Osirix using the Inspector. This demonstrates that the images are nearly the same dimensions in horizontal and vertical pixels, however, they are slightly different sizes. Because of this, the stack of images cannot be aligned pixel by pixel from one image to the next. The different image sizes are seen in the following screen capture. Using a graphical editing program, such as Seashore for the Mac, which is free, the images were resized so that all of them would be 605 x 455 pixels.
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